"Title Screen (Twilight Mix) - My Little Pony: Friendship Is Magic" is a high quality rip of the Twilight mix of the title screen theme from the game My Little Pony: Friendship Is Magic.
Following the joke from the previous My Little Pony rip, this rip uses the song "Pony" by Ginuwine. "Pony" is mashed up with "Kyun! Vampire Girl". From 2:15 onwards, the beat repeats until the rip fades to an end.